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Analytical Method Development and Validation for Quantitative Estimation of Torsemide in Bulk and Pharmaceutical Dosage Form by RP-HPLC

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Original Article

Author Details : Amol. Y. Ghodke, Bhagwat. N. Poul, Mohini. B. Sorde

Volume : 1, Issue : 1, Year : 2014

Article Page : 6-13

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Abstract

  

A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative estimation of Torsemide in Bulk and Pharmaceutical dosage form. The proposed RP-HPLC method was carried out on Zorbax C18 (250x4.6mm), 5?m column with mobile phase phosphate buffer and methanol (50:50) (v/v). The pH of phosphate buffer was adjusted by Ortho- phosphoric acid to 3.5. The flow rate was 1.3 mL/min and the detection wavelength was 288nm. The retention time of torsemide was found at 6.0±0.2min. The method was validated for specificity, precision, accuracy, linearity and robustness. The linearity range was 10-30 ?g/mL and correlation coefficient (r²) was found to be 0.9980. The mean % recovery for Torsemide was found to be 99.80. The developed method could be employed for the routine analysis of Torsemide from different formulations and for the Torsemide calculations as well.

 

How to cite : Ghodke A Y, Poul B N, Sorde M B, Analytical Method Development and Validation for Quantitative Estimation of Torsemide in Bulk and Pharmaceutical Dosage Form by RP-HPLC. Int J Pharm Chem Anal 2014;1(1):6-13

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