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Analytical method development and validation of UV-visible spectrophotometric method for the estimation of CNS stimulant drug in bulk

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Original Article

Author Details : Saba Khan*, Mohd Anas Shaikh, Nihal Shaikh, Jaya Agnihotri

Volume : 10, Issue : 2, Year : 2023

Article Page : 133-136

https://doi.org/10.18231/j.ijpca.2023.023

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Abstract

A simple, rapid, accurate, economical and precise UV/VIS method has been developed and validated. Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile, and 0.1 N HCL, and various concentrations ranges of various buffers were analyzed. Hence 0.1 N HCL was selected as a solvent for the proposed method. Caffeine showed maximum absorbance at 273nm.Drug obey Beer Lambert’s law in the concentration range of 3-18 ?g/mL for Caffeine. The LOD and LOQ were found to be 0.5476 ?g/mL and 1.6594 ?g/mL for Caffeine. The method was quantitatively evaluated in terms of linearity, precision, precision, LOD, LOQ and recovery. The method is simple, convenient and suitable for the analysis of Caffeine in bulk drugs.

Keywords: Caffeine, Precision, Accuracy, Method development

How to cite : Khan S, Shaikh M A, Shaikh N, Agnihotri J, Analytical method development and validation of UV-visible spectrophotometric method for the estimation of CNS stimulant drug in bulk. Int J Pharm Chem Anal 2023;10(2):133-136

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