Phyto nanoparticles, synthesized using plant-based materials, represent a transformative avenue in the field of nanomedicine. Leveraging their biocompatibility, low toxicity, and eco-friendly synthesis, these nanoparticles have emerged as versatile tools in drug delivery, bioimaging, diagnostics, and therapeutic interventions. This review delves into their applications in treating cancer, infectious diseases, inflammatory disorders, and other health conditions. It also explores their mechanisms of action, advantages over conventional nanomaterials, and potential challenges in clinical translation. By integrating green chemistry principles with cutting-edge biomedical strategies, Phyto nanoparticles hold the promise of addressing current healthcare challenges and improving patient outcomes.